cdna synthesis kit superscript iii first-strand synthesis system (Thermo Fisher)
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cdna synthesis kit superscript iii first-strand synthesis system
Cdna Synthesis Kit Superscript Iii First Strand Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+iii+first+strand+cdna+synthesis+kit/high+capacity+cdna+reverse+transcription+kit/pmc09483110__mmc3-158-10-18
Average 90 stars, based on 1 article reviews
Cdna Synthesis Kit Superscript Iii First Strand Synthesis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+iii+first+strand+cdna+synthesis+kit/high+capacity+cdna+reverse+transcription+kit/pmc09483110__mmc3-158-10-18
Average 90 stars, based on 1 article reviews
cdna synthesis kit superscript iii first-strand synthesis system - by Bioz Stars,
2026-09
90/100 stars
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Staining:Article Title: Electrical synapse structure requires distinct isoforms of a postsynaptic scaffold. Article Snippet: .. Stained tissue was either mounted (whole mount) and imaged in 75% glycerol, or dissected and mounted with ProLong Gold Antifade. qPCR analysis RNA was isolated from 5 dpf larvae using TRIzol reagent, and 5 μg total RNA was used to synthesize cDNA using the Real-time Polymerase Chain Reaction:Article Title: Electrical synapse structure requires distinct isoforms of a postsynaptic scaffold. Article Snippet: .. Stained tissue was either mounted (whole mount) and imaged in 75% glycerol, or dissected and mounted with ProLong Gold Antifade. qPCR analysis RNA was isolated from 5 dpf larvae using TRIzol reagent, and 5 μg total RNA was used to synthesize cDNA using the Article Title: Neuronal growth regulator 1 (NEGR1) promotes the synaptic targeting of glutamic acid decarboxylase 65 (GAD65). Article Snippet: Abbreviations: AgRP, agoutirelated peptide (AgRP); AQUA, advanced quick assembly; ARC, arcuate nucleus; CHO cell, Chinese hamster ovary cell; DNA, deoxyribonucleic acid; DTT, dithiothreitol; EDTA, ethylenediaminetetraacetic acid; EGTA, ethylene glycolbis(βaminoethyl ether)N,N,N′,N′tetraacetic acid; FGFR2, fibroblast growth factor receptor 2; GABA, γamino butyric acid; GAD65, glutamic acid decarboxylase 65 kDa isoform; GAD65membr, plasma membrane attached GAD65; GAD67, glutamic acid decarboxylase 67 kDa isoform; GAPDH, glyceraldehyde 3phosphate dehydrogenase; GFP, green fluorescent protein; GM130, 130 kDa cisGolgi matrix protein 1; GO, gene ontology; GPI, glycosylphosphatidylinositol; HA tag, Human influenza hemagglutininderived tag; HBS, HEPESbuffered saline; HEPES, 4(2hydroxyethyl)1piperazineethanesulfonic acid; HIP14, huntingtininteracting protein 14; HPLC, highperformance liquid chromatography; HRP, horseradish peroxidase; IC, immunocytochemistry; Ig, immunoglobulin; LCK, lymphocytespecific protein tyrosine kinase; MAP 2, microtubule associated protein 2; MFI, mean fluorescence intensity; mRNA, messenger ribonucleic acid; NEGR1, neuronal growth regulator 1; NPY, neuropeptide Y (NPY); PBS, phosphatebuffered saline; PMSF, phenylmethylsulfonyl fluoride; PVDF, polyvinylidene difluoride; RFP, red fluorescent protein; RNA, ribonucleic acid; RNAseq, RNA sequencing; VC tag, Cterminal amino acids 158–238 of the Venus fluorescent protein; VGAT, vesicular GABA transporter; VN tag, Nterminal amino acids 1–172 of the Venus fluorescent protein; WB, western blot.. 1School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales, Australia 2J.. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, USA 3School of Psychology, The University of New South Wales, Sydney, New South Wales, Australia Isolation:Article Title: Electrical synapse structure requires distinct isoforms of a postsynaptic scaffold. Article Snippet: .. Stained tissue was either mounted (whole mount) and imaged in 75% glycerol, or dissected and mounted with ProLong Gold Antifade. qPCR analysis RNA was isolated from 5 dpf larvae using TRIzol reagent, and 5 μg total RNA was used to synthesize cDNA using the Article Title: Retraction Notice: Neurospheres Induced from Human Adipose-Derived Stem Cells as a New Source of Neural Progenitor Cells. Article Snippet: After labeling, nuclei were stained with Dapi for 5 minutes and then the slides were immediately examined on a three-color immunofluorescence microscope (Nikon Instruments Inc., Tokyo, Japan). .. RNA was isolated using Trizol reagent (Invitrogen, Carlsbad, CA, USA). cDNA was transcribed using cDNA Synthesis:Article Title: Electrical synapse structure requires distinct isoforms of a postsynaptic scaffold. Article Snippet: .. Stained tissue was either mounted (whole mount) and imaged in 75% glycerol, or dissected and mounted with ProLong Gold Antifade. qPCR analysis RNA was isolated from 5 dpf larvae using TRIzol reagent, and 5 μg total RNA was used to synthesize cDNA using the Article Title: Retraction Notice: Neurospheres Induced from Human Adipose-Derived Stem Cells as a New Source of Neural Progenitor Cells. Article Snippet: After labeling, nuclei were stained with Dapi for 5 minutes and then the slides were immediately examined on a three-color immunofluorescence microscope (Nikon Instruments Inc., Tokyo, Japan). .. RNA was isolated using Trizol reagent (Invitrogen, Carlsbad, CA, USA). cDNA was transcribed using Article Title: Effect of propranolol on temporomandibular joint pain in repeatedly stressed rats. Article Snippet: Funding information Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Abstract Stress substantially increases the risk of developing painful temporomandibular disorders (TMDs) by influencing the release of endogenous catecholamines.. Propranolol, an antagonist of β-adrenergic receptors, has shown potential in alleviating TMD-associated pain, particularly when the level of catecholamines is elevated.. The aim of this study was to explore whether intra-articular propranolol administration is effective in diminishing temporomandibular joint (TMJ) pain during repeated stress situations. Article Title: Gold nanoparticle-mediated gene delivery induces widespread changes in the expression of innate immunity genes Article Snippet: RT-qPCR gene expression analysis RT-qPCR analysis for selected genes ( Supplementary Table S1 ) was performed using Taqman RNA assays (Applied Biosystems), according to standard protocols. .. Total RNA was reverse transcribed with random hexamers using a Article Title: Ex.50.T aptamer impairs tumor-stroma cross-talk in breast cancer by targeting gremlin-1. Article Snippet: .. RNA was retrotranscribed with Article Title: Cloning, characterization, and spatio-temporal expression patterns of HdhSPARC and its responses to multiple stressors Article Snippet: .. First-strand cDNA synthesis was conducted from the extracted total RNA using oligo(dt) primers (Sigma) and a Article Title: Methyl Paraben Affects Porcine Oocyte Maturation Through Mitochondrial Dysfunction Article Snippet: COCs were cultured in IVM medium (M199 medium containing 0.022 mg/mL sodium pyruvate, 10% porcine follicular fluid, 0.09 mg/mL L-cysteine, 1% penicillin-streptomycin, 10 IU/mL follicle-stimulating hormone, 20 ng/mL epidermal growth factor, and 10 IU/mL LH) for 44–46 h. Total RNA was isolated from the oviductal oocyte complex using the Dynabeads mRNA Direct Purification Kit (Invitrogen, Carlsbad, CA, USA). .. RNA was reverse-transcribed to cDNA using the Article Title: Neuronal growth regulator 1 (NEGR1) promotes the synaptic targeting of glutamic acid decarboxylase 65 (GAD65). Article Snippet: Abbreviations: AgRP, agoutirelated peptide (AgRP); AQUA, advanced quick assembly; ARC, arcuate nucleus; CHO cell, Chinese hamster ovary cell; DNA, deoxyribonucleic acid; DTT, dithiothreitol; EDTA, ethylenediaminetetraacetic acid; EGTA, ethylene glycolbis(βaminoethyl ether)N,N,N′,N′tetraacetic acid; FGFR2, fibroblast growth factor receptor 2; GABA, γamino butyric acid; GAD65, glutamic acid decarboxylase 65 kDa isoform; GAD65membr, plasma membrane attached GAD65; GAD67, glutamic acid decarboxylase 67 kDa isoform; GAPDH, glyceraldehyde 3phosphate dehydrogenase; GFP, green fluorescent protein; GM130, 130 kDa cisGolgi matrix protein 1; GO, gene ontology; GPI, glycosylphosphatidylinositol; HA tag, Human influenza hemagglutininderived tag; HBS, HEPESbuffered saline; HEPES, 4(2hydroxyethyl)1piperazineethanesulfonic acid; HIP14, huntingtininteracting protein 14; HPLC, highperformance liquid chromatography; HRP, horseradish peroxidase; IC, immunocytochemistry; Ig, immunoglobulin; LCK, lymphocytespecific protein tyrosine kinase; MAP 2, microtubule associated protein 2; MFI, mean fluorescence intensity; mRNA, messenger ribonucleic acid; NEGR1, neuronal growth regulator 1; NPY, neuropeptide Y (NPY); PBS, phosphatebuffered saline; PMSF, phenylmethylsulfonyl fluoride; PVDF, polyvinylidene difluoride; RFP, red fluorescent protein; RNA, ribonucleic acid; RNAseq, RNA sequencing; VC tag, Cterminal amino acids 158–238 of the Venus fluorescent protein; VGAT, vesicular GABA transporter; VN tag, Nterminal amino acids 1–172 of the Venus fluorescent protein; WB, western blot.. 1School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales, Australia 2J.. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, USA 3School of Psychology, The University of New South Wales, Sydney, New South Wales, Australia SYBR Green Assay:Article Title: Electrical synapse structure requires distinct isoforms of a postsynaptic scaffold. Article Snippet: .. Stained tissue was either mounted (whole mount) and imaged in 75% glycerol, or dissected and mounted with ProLong Gold Antifade. qPCR analysis RNA was isolated from 5 dpf larvae using TRIzol reagent, and 5 μg total RNA was used to synthesize cDNA using the Article Title: Ex.50.T aptamer impairs tumor-stroma cross-talk in breast cancer by targeting gremlin-1. Article Snippet: .. RNA was retrotranscribed with Article Title: Neuronal growth regulator 1 (NEGR1) promotes the synaptic targeting of glutamic acid decarboxylase 65 (GAD65). Article Snippet: Abbreviations: AgRP, agoutirelated peptide (AgRP); AQUA, advanced quick assembly; ARC, arcuate nucleus; CHO cell, Chinese hamster ovary cell; DNA, deoxyribonucleic acid; DTT, dithiothreitol; EDTA, ethylenediaminetetraacetic acid; EGTA, ethylene glycolbis(βaminoethyl ether)N,N,N′,N′tetraacetic acid; FGFR2, fibroblast growth factor receptor 2; GABA, γamino butyric acid; GAD65, glutamic acid decarboxylase 65 kDa isoform; GAD65membr, plasma membrane attached GAD65; GAD67, glutamic acid decarboxylase 67 kDa isoform; GAPDH, glyceraldehyde 3phosphate dehydrogenase; GFP, green fluorescent protein; GM130, 130 kDa cisGolgi matrix protein 1; GO, gene ontology; GPI, glycosylphosphatidylinositol; HA tag, Human influenza hemagglutininderived tag; HBS, HEPESbuffered saline; HEPES, 4(2hydroxyethyl)1piperazineethanesulfonic acid; HIP14, huntingtininteracting protein 14; HPLC, highperformance liquid chromatography; HRP, horseradish peroxidase; IC, immunocytochemistry; Ig, immunoglobulin; LCK, lymphocytespecific protein tyrosine kinase; MAP 2, microtubule associated protein 2; MFI, mean fluorescence intensity; mRNA, messenger ribonucleic acid; NEGR1, neuronal growth regulator 1; NPY, neuropeptide Y (NPY); PBS, phosphatebuffered saline; PMSF, phenylmethylsulfonyl fluoride; PVDF, polyvinylidene difluoride; RFP, red fluorescent protein; RNA, ribonucleic acid; RNAseq, RNA sequencing; VC tag, Cterminal amino acids 158–238 of the Venus fluorescent protein; VGAT, vesicular GABA transporter; VN tag, Nterminal amino acids 1–172 of the Venus fluorescent protein; WB, western blot.. 1School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales, Australia 2J.. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, USA 3School of Psychology, The University of New South Wales, Sydney, New South Wales, Australia Reverse Transcription:Article Title: Effect of propranolol on temporomandibular joint pain in repeatedly stressed rats. Article Snippet: Funding information Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Abstract Stress substantially increases the risk of developing painful temporomandibular disorders (TMDs) by influencing the release of endogenous catecholamines.. Propranolol, an antagonist of β-adrenergic receptors, has shown potential in alleviating TMD-associated pain, particularly when the level of catecholamines is elevated.. The aim of this study was to explore whether intra-articular propranolol administration is effective in diminishing temporomandibular joint (TMJ) pain during repeated stress situations. Article Title: Gold nanoparticle-mediated gene delivery induces widespread changes in the expression of innate immunity genes Article Snippet: RT-qPCR gene expression analysis RT-qPCR analysis for selected genes ( Supplementary Table S1 ) was performed using Taqman RNA assays (Applied Biosystems), according to standard protocols. .. Total RNA was reverse transcribed with random hexamers using a Article Title: Methyl Paraben Affects Porcine Oocyte Maturation Through Mitochondrial Dysfunction Article Snippet: COCs were cultured in IVM medium (M199 medium containing 0.022 mg/mL sodium pyruvate, 10% porcine follicular fluid, 0.09 mg/mL L-cysteine, 1% penicillin-streptomycin, 10 IU/mL follicle-stimulating hormone, 20 ng/mL epidermal growth factor, and 10 IU/mL LH) for 44–46 h. Total RNA was isolated from the oviductal oocyte complex using the Dynabeads mRNA Direct Purification Kit (Invitrogen, Carlsbad, CA, USA). .. RNA was reverse-transcribed to cDNA using the Article Title: Neuronal growth regulator 1 (NEGR1) promotes the synaptic targeting of glutamic acid decarboxylase 65 (GAD65). Article Snippet: Abbreviations: AgRP, agoutirelated peptide (AgRP); AQUA, advanced quick assembly; ARC, arcuate nucleus; CHO cell, Chinese hamster ovary cell; DNA, deoxyribonucleic acid; DTT, dithiothreitol; EDTA, ethylenediaminetetraacetic acid; EGTA, ethylene glycolbis(βaminoethyl ether)N,N,N′,N′tetraacetic acid; FGFR2, fibroblast growth factor receptor 2; GABA, γamino butyric acid; GAD65, glutamic acid decarboxylase 65 kDa isoform; GAD65membr, plasma membrane attached GAD65; GAD67, glutamic acid decarboxylase 67 kDa isoform; GAPDH, glyceraldehyde 3phosphate dehydrogenase; GFP, green fluorescent protein; GM130, 130 kDa cisGolgi matrix protein 1; GO, gene ontology; GPI, glycosylphosphatidylinositol; HA tag, Human influenza hemagglutininderived tag; HBS, HEPESbuffered saline; HEPES, 4(2hydroxyethyl)1piperazineethanesulfonic acid; HIP14, huntingtininteracting protein 14; HPLC, highperformance liquid chromatography; HRP, horseradish peroxidase; IC, immunocytochemistry; Ig, immunoglobulin; LCK, lymphocytespecific protein tyrosine kinase; MAP 2, microtubule associated protein 2; MFI, mean fluorescence intensity; mRNA, messenger ribonucleic acid; NEGR1, neuronal growth regulator 1; NPY, neuropeptide Y (NPY); PBS, phosphatebuffered saline; PMSF, phenylmethylsulfonyl fluoride; PVDF, polyvinylidene difluoride; RFP, red fluorescent protein; RNA, ribonucleic acid; RNAseq, RNA sequencing; VC tag, Cterminal amino acids 158–238 of the Venus fluorescent protein; VGAT, vesicular GABA transporter; VN tag, Nterminal amino acids 1–172 of the Venus fluorescent protein; WB, western blot.. 1School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales, Australia 2J.. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, USA 3School of Psychology, The University of New South Wales, Sydney, New South Wales, Australia Quantitative RT-PCR:Article Title: Ex.50.T aptamer impairs tumor-stroma cross-talk in breast cancer by targeting gremlin-1. Article Snippet: .. RNA was retrotranscribed with Article Title: Neuronal growth regulator 1 (NEGR1) promotes the synaptic targeting of glutamic acid decarboxylase 65 (GAD65). Article Snippet: Abbreviations: AgRP, agoutirelated peptide (AgRP); AQUA, advanced quick assembly; ARC, arcuate nucleus; CHO cell, Chinese hamster ovary cell; DNA, deoxyribonucleic acid; DTT, dithiothreitol; EDTA, ethylenediaminetetraacetic acid; EGTA, ethylene glycolbis(βaminoethyl ether)N,N,N′,N′tetraacetic acid; FGFR2, fibroblast growth factor receptor 2; GABA, γamino butyric acid; GAD65, glutamic acid decarboxylase 65 kDa isoform; GAD65membr, plasma membrane attached GAD65; GAD67, glutamic acid decarboxylase 67 kDa isoform; GAPDH, glyceraldehyde 3phosphate dehydrogenase; GFP, green fluorescent protein; GM130, 130 kDa cisGolgi matrix protein 1; GO, gene ontology; GPI, glycosylphosphatidylinositol; HA tag, Human influenza hemagglutininderived tag; HBS, HEPESbuffered saline; HEPES, 4(2hydroxyethyl)1piperazineethanesulfonic acid; HIP14, huntingtininteracting protein 14; HPLC, highperformance liquid chromatography; HRP, horseradish peroxidase; IC, immunocytochemistry; Ig, immunoglobulin; LCK, lymphocytespecific protein tyrosine kinase; MAP 2, microtubule associated protein 2; MFI, mean fluorescence intensity; mRNA, messenger ribonucleic acid; NEGR1, neuronal growth regulator 1; NPY, neuropeptide Y (NPY); PBS, phosphatebuffered saline; PMSF, phenylmethylsulfonyl fluoride; PVDF, polyvinylidene difluoride; RFP, red fluorescent protein; RNA, ribonucleic acid; RNAseq, RNA sequencing; VC tag, Cterminal amino acids 158–238 of the Venus fluorescent protein; VGAT, vesicular GABA transporter; VN tag, Nterminal amino acids 1–172 of the Venus fluorescent protein; WB, western blot.. 1School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales, Australia 2J.. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, USA 3School of Psychology, The University of New South Wales, Sydney, New South Wales, Australia |